Individually addressable arrays of replica microbial cultures enabled by splitting SlipChips

Individually addressable arrays of replica microbial cultures enabled by splitting SlipChips.

Isolating microbes carrying genes of interest from environmental samples is important for applications in biology and medicine. However, this involves the use of genetic assays that often require lysis of microbial cells, which is not compatible with the goal of obtaining live cells for isolation and culture. This paper describes the design, fabrication, biological validation, and underlying ph...

Parallel dip-pen nanolithography with arrays of individually addressable cantilevers

In dip-pen nanolithography ~DPN!, nanoscale chemical patterns are created by directly transferring chemical molecules from the tip of an atomic force microscope probe to a surface. We report the development of a thermally actuated probe array for DPN applications. The array consists of ten thermal bimorph actuated probes, each 300 mm long, with a lateral spacing of 100 mm. The probes are actuat...

Spatial Resolution of Single-Cell Exocytosis by Microwell-Based Individually Addressable Thin Film Ultramicroelectrode Arrays

We report the fabrication and characterization of microwell-based individually addressable microelectrode arrays (MEAs) and their application to spatially and temporally resolved detection of neurotransmitter release across a single pheochromocytoma (PC12) cell. The microwell-based MEAs consist of 16 4-μm-width square ultramicroelectrodes, 25 3-μm-width square ultramicroelectrodes, or 36 2-μm-w...

Parallel transport of biological cells using individually addressable VCSEL arrays as optical tweezers

We have demonstrated the use of vertical cavity surface emitting lasers (VCSELs) for optical trapping and active manipulation of live biological cells and microspheres. We have experimentally verified that the Laguerre–Gaussian laser mode output from the VCSEL functions just as well as the traditional Gaussian fundamental laser mode for optically trapping biological cells and may be preferable ...

Biosensing at an Individually Addressable Electrochemical Array